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Please use this identifier to cite or link to this item:
http://hdl.handle.net/2282/825
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| Title: | Detection of tick-borne pathogens by molecular methods |
| Authors: | Paulauskas, Algimantas
Radzijevskaja, Jana
Ambrasiene, Daiva
Rosef, Olav |
| Issue Date: | 2008 |
| Publishers version: | http://dx.doi.org/10.2478/v10054-008-0040-6 |
| Abstract: | The use of molecular methods such as species-specific PCR, Multiplex-PCR, RT-PCR, reverse
line blot hybridisation in investigations of tick-borne pathogens allowed to detect and identify
the causative agents of Lyme borreliosis, anaplasmosis, and babesiosis in ticks and rodents in
Lithuania and Norway. The overall prevalence of Borrelia burgdorferi s. l. infection detected in
Lithuanian and Norwegian ticks was found to be 13.3% (223/1679) and 5.4% (75 /1383), respectively.
A total 68 of 398 (17.1%) rodent ear extractions screened by PCR were found positive for
B. burgdorferi s. l. infection. B. burgdorferi s. l. was detected in 53 of 428 (12.4%) immature Ixodes
ricinus ticks collected on rodents in Lithuania and in 30 of 782 (3.8%) collected on rodents in
Norway. In 24 of 173 (13.8%) ticks feeding on passerine migrating birds collected in Norway,
B. burgdorferi s. l. pathogens were detected. Three clinically important species (B. afzelii, B. garinii
and B. burgdorferi s. s.) were identified in ticks and rodents. Anaplasma sp. was detected in
5% of questing ticks and 19.6% of ticks collected from birds in Lithuania. A. phagocytophilum
pathogens were detected in 7.1% of ticks from birds and in 93 of 1634 (5.7%) I. ricinus ticks collected
from vegetation in Norway. To Babesia divergens, positive were 2% and 0.9% of questing
ticks collected in Lithuania and Norway respectively. |
| Keywords: | Tick-borne pathogens
Ixodes ricinus
PCR
Molecular detection methods
Ticks |
| Publisher: | Lithuanian Academy of Sciences Publishers |
| Document type: | Journal article |
| URI: | http://hdl.handle.net/2282/825 |
| Appears in Collections: | Institutt for natur-, helse- og miljøvernfag
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