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Please use this identifier to cite or link to this item:
http://hdl.handle.net/2282/1259
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| Title: | Evaluation of in-field DNA degradation of mitochondrial- and genomic DNA in snowsampled faecal pellets from rock ptarmigan (Lagopus muta) |
| Authors: | Bergan, Frode |
| Issue Date: | 2011 |
| Abstract: | The use of non invasive genetic sampling (NGS) have become increasingly relevant in genetic
studies applied to ecology. Reliable methods should be tailored to species and environments.
Rock ptarmigan (Lagopus muta) spend their entire lifecycle in the alpine region and roost in
snow holes to save energy and avoid predation. Faecal pellets (FP) were collected from 20
individual rock ptarmigan snow roosts in the Lifjell mountain area in Telemark, Norway
repeatedly between 17 january and 7 july, 2010. DNA was extracted from 146 samples, and
degradation evaluated in PCR products of mitochondrial DNA (mtDNA), and 3 genomic
DNA (nDNA) microsatellites. For mtDNA, the mean duration in 20 roosts was 73.8 and 59.4
days respectively in a best and worst case scenario. For the 3 nDNA microsatellites, mean
duration times varied between 38.8 - 58.1 and 20.9 - 45.8 days respectively in a best and
worst case scenario. MtDNA had a significantly longer duration than nDNA, and duration
times between individual nDNA microsatellites varied significantly. Overall PCR error rates
in the study period were 0.48 for mtDNA and 0.53 - 0.68 for 3 nDNA microsatellites. DNA
degradation appeared to accelerate considerably when frozen, snow covered FPs eventually
became exposed to higher temperatures and precipitation during the spring snow-melt. FPs
from rock ptarmigan snow roosts serves as a promising DNA source in genetic studies, and
method may be applied to other snowroosting grouse. |
| Keywords: | Fjellryper |
| Publisher: | Høgskolen i Telemark |
| Document type: | Master thesis |
| Peer reviewed: | Not peer reviewed |
| URI: | http://hdl.handle.net/2282/1259 |
| Rights: | © Copyright The Author. All rights reserved |
| Bibliographic citation: | Bergan, F. Evaluation of in-field DNA degradation of mitochondrial- and genomic DNA in snowsampled faecal pellets from rock ptarmigan (Lagopus muta). Master thesis, Telemark University College, 2011 |
| Appears in Collections: | Mastergradsavhandlinger i natur-, helse- og miljøvern
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